

Brand: Thermo
Model: Attune NxT
Fixed Asset Number: S220002190
Affiliated Core: Biochemistry Core
Location: 107,Building A, Gaoke Innovation Center
Equipment Manager: Li Mengyuan
Contact: 13368393404, limy@szbl.ac.cn
Four lasers with 14 colors, allowing for multi - color cell analysis.
By rapidly stimulating particle signals in sheath fluid, various components and functional states can be qualitatively and quantitatively analyzed, including:
- Cell surface marker detection: For immunophenotyping and analysis of other surface proteins.
- Intracellular protein detection: Including cytokines, cytoskeletal molecules and cytoplasmic enzymes.
- Intracellular ion concentration detection: Such as H+, Na+, K+ , Ca2+ , etc.
- Nuclear component detection: Covering cell cycle and DNA content analysis.
- Cell membrane: Fluidity, membrane potential and permeability.
- Cell function: Such as viability assessment, pH value, proliferation/apoptosis and drug resistance.
- Gene expression: Detection of endogenous and exogenous gene expression, fluorescence reporter gene expression and transfection efficiency.
Before loading samples, filter the cells using a 40μm nylon mesh or 300 - mesh nylon screen. Adjust the cell density to approximately 1 - 10×106 cells/ml, and ensure the sample volume is no less than 200μL.