Biomolecular layer interferometry (BLI) is a label - free technology that converts the optical interference signals occurring on the surface of a BLI biosensor into real - time response signals. By real - time monitoring of molecular binding processes, the system measures binding constants (kon or ka) and dissociation constants (koff or kd), as well as the initial binding rate. Then, it calculates and analyzes the affinity (KD) and concentration information through fitting.

Comprehensive analysis of protein - protein interaction: such as the functions of proteins in proteomics, signal conduction in signaling pathways, etc.
Research on the mechanism of cancer: directly analyze complex samples like clinical specimens, tissues, and cell homogenates, and screen and identify tumor markers.
Screening and identification of small - molecule drugs and natural products of traditional Chinese medicine: rapidly and accurately screen small - molecule drugs that interact with target proteins.
Antibody screening: used for antibody screening in antibody libraries or epitope pairing of polyclonal antibodies, especially for low - affinity antibodies or those with very fast dissociation.
Analysis of interactions between nucleic acids (DNA/RNA) and proteins: such as gene regulation and transcription mechanism research.
Interactions between viral particles and proteins, etc.